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Perl's stain for Iron

Container:

Perl's stain for Iron
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Reference Range:

See below

Turnaround Time:

24 hours

Description:

Perls' stain (Prussian-Blue Reaction) is to demonstrate Ferric salts in tissues. These are seen as iron granules in bone marrow macrophages, erythroblasts (Sideroblasts) and erythrocytes (Siderocytes) in blood films and haemosiderin in spun urine. Bone marrow and blood smears are prepared and fixed in methanol Urine is centrifuged , supernatant discarded and slides made from the concentrate and fixed.Blood and bone marrow films can be stained by the Perl’s reaction for free and intracellular iron. This method is useful in the classification of forms of Anaemia. Ionised iron in presence of Hydrochloric acid reacts with acidified ferrocyanide to give a blue-green colour.

Preparation:

Bone Marrow or Peripheral blood smears - Slides are fixed in methanol for 15 minutes. Make sure that bone marrow slides have at least one particle present

Vessels and Containers:

1 x04.5 ml EDTA (Purple Top) or Bone marrow smears

Sample Requirements:

Bone Marrow or Peripheral blood smears - Slides are fixed in methanol for 15 minutes. Make sure that bone marrow slides have at least one particle present

How result is reported:

Granules containing iron will stain bright blue in marrow films. Siderotic granules will appear in the red cells of patients with sidreoblastic anaemia. Reporting - The films are reported in conjunction with the bone marrow aspiration by a medical officer.

Important Information:

Iron stains on Bone Marrow will only be requested by a Consultant Haematologist