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ENA (Extractable Nuclear Antigen) Antibodies (Ro, La, Sm, RNP, Scl-70, Jo-1)


ENA (Extractable Nuclear Antigen) Antibodies (Ro, La, Sm, RNP, Scl-70, Jo-1)
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Reference Range:

Normal Result = Negative

Turnaround Time:

5 working days


Internal Test Code:- ENAS


Indication: Connective tissue disease, systemic lupus erythematosus (SLE), Sjogren's syndrome

Background:These recognise saline-extracted cell antigens, from both nucleus and cytoplasm. Used in the classification of connective tissue diseases. Patients with SLE or Sjogren’s Syndrome should be screened for ENA antibodies, especially if they are considering pregnancy. Absence of antibody does not exclude a clinical diagnosis. No correlation with anti-dsDNA antibodies. An initial screen is performed. If positive, ENA typing will be performed.

Specificity & Disease Association
Anti-Ro (Anti-SS-A) - SLE; Cutaneous LE; Sjogren’s Syndrome; Recurrent Miscarriage; Neonatal Lupus; Congenital Heart Block
Anti-La (Anti-SS-B) - SLE; Sjogren’s Syndrome
Anti-Sm - SLE (common only in West Indians)
Anti-RNP - SLE; Mixed Connective Tissue Disease
Anti-Scl-70 - Systemic sclerosis
Anti-Jo-1 - Polymyositis; Dermatomyositis

Method: Enzyme Linked Immunosorbent Assay (ELISA)

Repeat Frequency: Typing should be performed twice (for confirmation) at least three months apart. Subsequent requests where type has been confirmed will only have screen performed and comment regarding type added.



Vessels and Containers:

Serum gold-top

Sample Requirements:

250µl serum


Grossly haemolysed or lipaemic samples should be avoided.

How result is reported:

Reported as - Negative or Positive

Important Information:

Entry Last Modified 14/03/2019

Transportation and Handling:

At room temperature

Special Precautions:


Time limits for requesting additional examinations:

Sample available in this laboratory for approximately 2 weeks. Test can only be added to samples already received by the immunology laboratory and within this time period.